Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization
Abstract HIV-1 Nef is an important pathogenic factor for HIV/AIDS pathogenesis.Studies have shown that the association of Nef hard steel shot with the inner leaflet of the plasma membrane and with endocytic and perinuclear vesicles is essential for most activities of Nef.Using purified recombinant proteins in pull-down assays and by co-immunoprecip